Mossing lab DNA sequences.
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Cloning Vectors
- pUC18.fasta Here is a link to the official pUC18 genbank entry at NCBI.
- pUC118.fasta Official pUC118 genbank entry at NCBI.
- pET21aPDF Map from Novagen
- pET33bPDF Map from Novagen
Cro Expression plasmids
- Here is a graphical genealogy Cro Plasmid Maps (PDF) of Cro expression plasmids from the Sauer Lab
- pUCroRS.fasta and Reverse Complement (pUCroRSrc.fasta) EcoRI - Tac promoter and synthetic Cro gene - (SmaI) of pAP120 inserted in pUC18. Interactive HTML from sequence extractor.
- Cro substitution mutant - tryptophan for phenylalanine at 58 Reverse Complement pUCroF58W_RSrc.fasta
- pUC118CroRX like pUCroRS but includes terminator from pAP120. This PDF file shows the difference.
- pUC119CroRX like pUC118CroRX but EcoR1 to hindIII segment is reversed. This PDF file shows the difference.
- pUCromDG.fasta like pUCroRs but contains a small insertion in the Cro gene. Mossing & Sauer Science (1990)
- Cro K56[DGEVK] F58W fragment (EcoRI) - Tac promoter and synthetic Cro monomer gene.
- pJF1a Cro in pET21a with C-terminal His 6 fusion (91 Amino Acids)
- pscCro8 single chain dimer, 8 residue linker in pET21a (reverse complement) Interactive HTML from sequence extractor.
- pscCro16 single chain dimer, 16 residue linker in pET21a (reverse complement).
- pET33bscCro8.fasta Reverse complement of pET33b sequence above with scCro 8 sequence cloned between NdeI and XhoI sites.
Reporter constructs
- pucOR_Cro lambda OR-Olac control region replaces the Tac promoter of puCroRS
- pUCOR_Cro_lac same as pucOR_Cro but with translation start of lac Zalpha reconstructed. Here is an annotated plain text sequence file of the important parts.
- pAH125 (PDF summary pAH125.pdf | genbank sequencepAH125.genbank | ncbi link) is a promoterless lacZ reporter plasmid, with kanR, OriR and Att lambda for generation of single copy integrants
- pUA139 is similar to pMS201 (Map and sites pMS201.html | genbank sequence pMS201.genbank) except BamHI and XhoI sites upstream of GFPmut2 are reversed (Bam-Xho-GFP), kanR and pSC101 origin
- pUAL306 (guesstimate PDF summary pUALuxBX.pdf | guesstimate fasta sequence pUALuxBX.fasta ) BamHI and XhoI sites upstream of has luxCDABE kanR and pSC101 origin
- Hybrid PR-lacO promoter oligonucleotides PR-lacO_oligos.txt
- New plasmid constructed by Yin Gu from pUA139 and Hybrid PR-lacO promoter oligonucleotides pOR1GFP.fasta.
- Raw sequence data from 9/8/05.
- pOR1GFP + scCro DNA pOR1scCroGFP.fasta.
- pOR1GFP + scCro DNA reversed pOR1scCro_RGFP.fasta.
- pAH125 Pst-Sal-lacI(rc)-Sph-OPO-Xba..-EcoRI-BamHI-lacZ pAH125laciOPO.fasta. lacI (rev. comp.) + lambda PR/lac O promoter inserted between the PstI and XbaI sites of pAH125 Fixed 2016.03.07
- pAH125 Pst-Sal-lacI(rc)-Sph-OPOXba-scCro-XhoI-EcoRI-BamHI-lacZ pAH125lacI_OPO_scCro.fasta.
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